引用本文:韩燚1,谢玮鑫1,肖洁2,陈雪梅2,李展春1.神经肽Y和受体在腰椎动静力失衡大鼠模型中表达及对痛阈的影响[J].重庆医科大学学报,2019,44(6):689~
神经肽Y和受体在腰椎动静力失衡大鼠模型中表达及对痛阈的影响
Expression of neuropeptide Y and its receptors in the rat model of intervertebral disc degeneration induced by unbalanced dynamic and static forces and its correlation with pain threshold
DOI:
中文关键词:  神经肽Y  动静力失衡  椎间盘源性腰痛  疼痛行为学
英文关键词:neuropeptide Y  unbalanced dynamic and static forces  discogenic low back pain  pain behavior
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韩燚1,谢玮鑫1,肖洁2,陈雪梅2,李展春1 上海交通大学医学院附属仁济医院1. 骨科2. 麻醉科上海 200127 
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中文摘要:
      目的:观察神经肽Y(neuropeptide Y,NPY)及Y1R、Y2R在椎间盘退变 (intervertebral disc degeneration,IDD)组和假手术(sham operation,SHAM)组大鼠椎间盘(intervertebral disc,IVD)和背根神经节(dorsal root ganglion,DRG)表达的差异,以及与大鼠机械缩足阈值(mechanical withdrawal threshold,MWT)和热缩足反射潜伏期(thermal withdrawal latency,TWL)的相关关系,探讨NPY及受体与椎间盘源性腰痛的关系。方法:选择6月龄无特定病原体(specific pathogen free,SPF)级雌性未孕SD大鼠12只,随机分为椎间盘退变组和假手术组(n=6),前者采用动静力失衡大鼠椎间盘退变模型,后者做假手术处理,造模后12周通过MRI和X线评估椎间盘退变模型建立是否成功并测量2组大鼠MWT及TWL,使用免疫组织化学法检测2组大鼠IVD和DRG中NPY及受体的表达,分析其平均光密度(mean optical density,MOD)值与疼痛行为学之间的相关性。结果:①疼痛检测发现,术后12周IDD组大鼠MWT较SHAM组大鼠明显降低[(9.44±2.91) g vs. (17.99±4.40) g,P=0.002],TWL明显降低[(9.17±0.39) s vs. (13.53±1.69) s,P=0.002]。②免疫组织化学法检测显示,与SHAM组相比,IDD组大鼠IVD和DRG中NPY的表达明显升高[IVD:[(0.15±0.03) vs. (0.11±0.02),P=0.021;DRG:(0.23±0.04) vs. (0.17±0.04),P=0.018],Y1R表达明显降低[IVD:(0.10±0.02) vs. (0.13±0.01),P=0.009;DRG:(0.05±0.01) vs. (0.08±0.01),P=0.000],2组大鼠IVD和DRG中Y2R的表达差异均无统计学意义。③Pearson相关性分析表明,2组大鼠IVD和DRG中NPY的MOD值与MWT之间成负相关关系(r=-0.605,P=0.037;r=-0.617,P=0.033);2组大鼠IVD和DRG中Y1R的MOD值与MWT之间成正相关关系(r=0.696,P=0.012;r=0.735,P=0.006)。2组大鼠IVD和DRG中NPY的MOD值与TWL之间成负相关关系(r=-0.603,P=0.038;r=-0.708,P=0.010);2组大鼠IVD和DRG中Y1R的MOD值与TWL之间成正相关关系(r=0.624,P=0.030;r=0.834,P=0.001)。结论:在腰椎动静力失衡大鼠模型中,NPY可能通过外周Y1R参与椎间盘源性腰痛的发生发展,但其具体机制有待进一步研究。
英文摘要:
      Objective:To investigate the expression of neuropeptide Y(NPY),Y1 receptor(Y1R),and Y2 receptor(Y2R) in the inter-vertebral disc(IVD) and dorsal root ganglion(DRG) of rats with intervertebral disc degeneration(IDD) and sham-operated rats,and to investigate the correlation of NPY and its receptors with mechanical withdrawal threshold(MWT) and thermal withdrawal latency (TWL) in rats and the relationship of NPY and its receptors with discogenic low back pain. Methods:Twelve 6-month-old female specific pathogen-free Sprague-Dawley rats which were not pregnant were equally and randomly divided into two groups:IDD group and sham-operation group. An IDD model was induced by un-balanced dynamic and static forces in the IDD group,and sham operation was performed in the sham-operation group. At 12 weeks after the operation,whether the establishment of the IDD model was successful was evaluated by magnetic resonance imaging and X-ray,and MWT and TWL were measured. The expression of NPY and its receptors in the IVD and DRG was determined by immunohistochemistry to analyze the correlation of the mean optical density(MOD) of NPY and its receptors with pain behaviors(MWT and TWL). Results:①Pain testing results showed that the IDD group had significantly lower MWT and TWL compared with the sham-operation group at 12 weeks after operation[(9.44±2.91) g vs. (17.99±4.40) g,P=0.002;(9.17±0.39) s vs. (13.53±1.69) s,P=0.002]. ②Immunohistochemical analysis showed that the IDD group had significantly higher expression of NPY in the IVD and DRG and significantly lower expression of Y1R in the IVD and DRG compared with the sham-operation group(IVD:0.15±0.03 vs. 0.11±0.02,P=0.021;DRG:0.23±0.04 vs. 0.17±0.04,P=0.018;IVD:0.10±0.02 vs. 0.13±0.01,P=0.009;DRG:0.05±0.01 vs. 0.08±0.01,P=0.000). There was no signifi-cant difference in the expression of Y2R in the IVD and DRG between the two groups(P>0.05). ③Pearson correlation analysis showed that the MOD values of NPY in the IVD and DRG were negatively correlated with MWT and TWL in the two groups(r=-0.605,P=0.037;r=-0.617,P=0.033;r=-0.603,P=0.038;r=-0.708,P=0.010);the MOD values of Y1R in the IVD and DRG were positively correlated with MWT and TWL in the two groups(r=0.696,P=0.012;r=0.735,P=0.006;r=0.624,P=0.030;r=0.834,P=0.001). Conclusion:In the rat model of unbalanced dynamic and static forces,NPY may be involved in the development and progression of discogenic low back pain through peripheral Y1R,but the specific mechanism needs further investigations.
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