引用本文:杨 桦.蒽环类药物耐药乳腺癌细胞中microRNA差异表达分析[J].重庆医科大学学报,2012,37(1):56~58
蒽环类药物耐药乳腺癌细胞中microRNA差异表达分析
Analysis of differential microRNA expression in anthracycline-resistant breast cancer cell lines
DOI:
中文关键词:  乳腺癌  耐药  microRNA
英文关键词:breast cancer  drug resistance  microRNA
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作者单位
杨 桦 河南省肿瘤医院乳腺科郑州 450003 
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中文摘要:
      目的:检测乳腺癌蒽环类药物耐药细胞株中微小RNA(microRNA,miRNA)的表达差异,探讨miRNA与乳腺癌化疗耐药的关系。方法:miRNA芯片检测乳腺癌蒽环类药物(阿霉素)耐药细胞MCF-7/ADM和其亲本细胞MCF-7的miRNA表达谱,找出差异表达的miRNA,并利用实时荧光RT-PCR法对miRNA芯片结果进行验证。结果:耐药细胞株MCF-7/ADM与亲本细胞株MCF-7相比有14个差异表达的miRNA,其中上调表达的有6个,下调表达的有8个;实时荧光RT-PCR进一步验证证实has-miR-221、has-miR-222、has-miR-106a在MCF-7/ADM细胞中表达显著上调,而has-let-7a、has-miR-421、has-miR-23b在MCF-7/ADM细胞中表达显著下调,与芯片的结果一致。结论:miRNA在乳腺癌蒽环类药物耐药细胞和其亲本细胞中差异表达,可能参与乳腺癌耐药的发生
英文摘要:
      Objective: To analyze the difference in microRNA expression in breast cancer cell lines of anthracycline anticancer drug resistance and explore the association between microRNA and drug resistance of breast cancer. Methods:The differentially expressed miRNAs between anthracycline anticancer drug-resistant breast cancer cell line MCF-7/ADM and its parental cell line MCF-7 were identified by miRNA profiling of these two cell lines using miRNA microarray;the results obtained by microarray profiling were vali-dated using real-time RT-PCR analysis. Results:Microarray analysis of MCF-7 to MCF-7/ADM cells identified 14 differentially ex-pressed genes,including 6 up-regulated and 8 down-regulated genes in MCF-7/ADM cells;real-time RT-PCR identified 6 microR-NAs that were differentially expressed between MCF-7 and MCF-7/ADM cells,including 3 up-regulated microRNAs:has-miR-221,has-miR-222,has-miR-106a,and 3 down-regulated microRNAs:has-let- 7a,has-miR -421,has-miR-23b in MCF-7/ADM cells.Conclusion:microRNA are differentially expressed in anthracycline anticancer drug-resistant breast cancer cell lines and its parental cell line,which suggests that microRNA may play a role in the development of drug-resistance in breast cancer cells.
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