引用本文:冯 渊,李德卫,杨晓波,陈 睿,杜文俊,樊 斌.经颈动脉途径肝动脉插管及脾内注射肝细胞移植治疗急性肝衰大鼠的疗效比较[J].重庆医科大学学报,2012,37(1):84~88
经颈动脉途径肝动脉插管及脾内注射肝细胞移植治疗急性肝衰大鼠的疗效比较
Comparison of therapeutic effects between catheterization of hepatic artery via carotid route and intrasplenic injection for hepatocyte transplantation in acute hepatic failure rats
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中文关键词:  肝动脉插管  脾脏内移植  肝细胞移植
英文关键词:catheterization hepatic artery  intrasplenic injec-tion  hepatocyte transplantation
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冯 渊,李德卫,杨晓波,陈 睿,杜文俊,樊 斌 重庆医科大学附属第一医院肝胆暨肝移植外科重庆 400016 
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中文摘要:
      目的:建立SD大鼠颈动脉途径肝动脉插管肝细胞移植的动物模型;探讨肝动脉与脾脏直接注射2种途径肝细胞移植治疗急性肝衰竭(Acute hepatic failure,AHF)大鼠的疗效。方法:经D-氨基半乳糖(D-galactosamine,D-gal)诱导大鼠AHF 24 h后,65只大鼠经颈动脉途径肝动脉插管,60只成功后分为3组,Ⅰ组(n=20)经脾脏直接注射约2×107 个肝细胞、肝动脉注射0.4 ml Hank’s液;Ⅱ组(n=20)经脾脏直接注射0.4 ml Hank’s液、肝动脉注射2×107 个肝细胞;Ⅲ组(n=20)经脾脏直接注射0.4 ml Hank’s液、肝动脉注射0.4 ml Hank’s液。观察每组大鼠的生存率及肝功能变化情况,追踪经肝动脉移植荧光示踪剂CFDA SE标记的肝细胞,HE染色观察脾内肝细胞病理变化和免疫荧光观察白蛋白分泌作用。结果:Ⅰ组14 d存活率显著高于Ⅱ组(P=0.031,<0.05),Ⅱ组14 d存活率也高于Ⅲ组(P=0.048,<0.005);Ⅰ、Ⅱ组都能明显改善AHF大鼠的肝功能,尤其以Ⅰ组最为显著;Ⅱ组CFDA SE荧光标记的肝细胞肝动脉移植24 h后,受体大鼠肝脏可以看到散在绿色荧光分布;Ⅰ组移植30 d后,白蛋白共焦免疫荧光结果表明,脾内有肝细胞白蛋白绿色荧光信号;Ⅰ组移植7 d后,HE染色可以看到肝细胞在脾脏红髓中簇集在一起并定植下来。结论:经大鼠颈动脉途径肝动脉插管肝细胞移植能提高AHF大鼠的存活率和改善其肝功能;脾脏直接注射肝细胞移植改善AHF大鼠生存率和肝功能更优于肝动脉途径。
英文摘要:
      Objective:To establish the animal model of hepatocyte transplantation via hepatic artery in SD rats;to evaluate the treat-ment effects of hepatocyte transplantation by hepatic artery and intrasplenic injection in rats with acute hepatic failure. Methods:A-cute hepatic failure(AHF) was induced by D-gal in SD rats. After 24 hours,a cather was inserted into the hepatic artery in 65 rats with AHF. Then the 60 rats were randomly divided into 3 groups. GroupⅠreceived 2×107 hepatocytes through intraplenic injection and 0.4 ml Hank’s solution was infused through hepatic artery;GroupⅡ received 0.4 ml Hank’s solution through intraplenic injection and 2×107 hepatocytes was infused through hepatic artery;Group Ⅲ received 0.4 ml Hank’s solution through intraplenic injection and 0.4 ml Hank’s solution through hepatic artery.Surviving rate was observed at 14 days and liver function was measured at different time points. The distribution of transplanted CFDA-SE-labeled hepatocytes through hepatic artery was observed.Secretion of albumin and liver histopathological change were observed in spleen by immunofluorescence and HE staining. Results:At 14 days,the survival of Group Ⅰrats was significantly higher than that of Group Ⅱ rats(P=0.031,<0.05);the survival of Group Ⅱwas significantly higher than that of Group Ⅲ(P=0.048,<0.05).Hepatic function of GroupsⅠand Ⅱhad improved,especially of GroupⅠ. In Group Ⅱ,24 h after transplantation of CFDA-SE-labeled hepatocytes,scattered green fluorescence region was found under fluorescent microscopy. In GroupⅠ,at 30 days post-transplatation,immunofluorescent staining of albumin demonstrated some positive cells in spleen;at 7 days post-transplatation,transplanted hepatocytes was found in spleen. Conclusion:Hepatocyte transplantation through catheteriza-tion hepatic artery via carotid route can improve the survival of rats with AHF and ameliorate hepatic function,but intrasplenic injec-tion is significantly superior to it.
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