引用本文:杨中玫,罗 欣,白 冰,漆洪波.KLF8在人早孕绒毛组织中的表达及其意义[J].重庆医科大学学报,2013,(10):1133~1137
KLF8在人早孕绒毛组织中的表达及其意义
Expression and significance of Krüppel-like factor 8 in human villous tissues during early pregnancy
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中文关键词:  早期妊娠  绒毛  滋养细胞  转录因子
英文关键词:early pregnancy  villi  trophoblast  transcriptional factor
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杨中玫,罗 欣,白 冰,漆洪波 重庆医科大学附属第一医院妇产科重庆 400016 
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中文摘要:
      目的:通过检测正常孕妇早孕期绒毛组织中双向转录因子Krüppel-like factor 8(KLF8)的定位及表达,探讨其在早孕期滋养细胞侵袭中的作用。方法:选择2012年3月至2012年6月在重庆医科大学附属第一医院门诊孕6~11周进行选择性流产的22例正常孕妇为研究对象,取绒毛组织标本,采用免疫组化SP法检测各组孕妇绒毛组织中KLF8蛋白的定位,实时荧光定量PCR技术检测各组孕妇绒毛组织中KLF8 mRNA表达水平,采用蛋白印迹法检测各组孕妇绒毛组织中KLF8、基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)蛋白的表达水平。结果:(1)正常孕6~11周绒毛组织中均有KLF8蛋白表达,主要定位于绒毛组织滋养层中的细胞滋养细胞和合体滋养细胞。(2)KLF8 mRNA 随妊娠周数增加而逐渐升高,并在孕8~10周达到高峰(孕8周最高),而后急剧降低。(3)正常早孕绒毛组织中KLF8、MMP-9蛋白表达水平同样随妊娠周数增加而逐渐升高,并在孕8~10周达到最高峰(孕9周最高),而后逐渐降低。(4)正常早孕绒毛组织中KLF8蛋白及MMP-9蛋白表达呈正相关关系,相关系数r为0.66(P<0.001)。结论:KLF8在正常早孕组织中定位于滋养层细胞尤其是与侵袭密切相关的细胞滋养细胞中,且表达变化与MMP-9蛋白水平以及滋养细胞浸润能力相吻合,提示KLF8可能参与胚胎植入及胎盘形成过程中滋养细胞浸润活性调控。
英文摘要:
      Objective:To evaluate the expression and localization of Krüppel-like factor 8(KLF8) in human villous tissues during ear-ly pregnancy and to explore the effect of KLF8 on trophoblast invasion during early pregnancy. Methods:A total of 22 early pregnan-cy human villous tissues of 6-11 weeks of gestation(weeks) were obtained from elective terminations of apparently normal pregnancies from March 2012 to June 2012 in The First Affiliated Hospital of Chongqing Medical University. And then immunohistochemical SP method was employed to detect the localization of KLF8 protein. KLF8 mRNA level was determined by quantitative real-time PCR technique and Western blot analysis was used to quantify KLF8 and matrix metalloproteinase-9(MMP-9) protein expression levels.Results:(1)There were KLF8 immunoreactivity expressed in villous tissues from 6-11 weeks. It was primarily located in cyto-and syn-cytio-trophoblast in the trophoblast layer of the chorionic villi. (2)KLF8 mRNA levels were gradually increased with gestational ages,peaked at 8-10 gestational ages(being the highest at 8 weeks) and then decreased sharply. (3)KLF8 and MMP-9 protein levels also shared the same pattern of expression in human villous tissues from early pregnancy:gradually increased from 6 weeks,peaked at 8-10 weeks(being the highest at 8 weeks) and then slightly decreased at the end of the first trimester(11 weeks). (4)There was a posi-tive correlation between the expression of KLF8 and MMP-9 protein in human villous tissues from early pregnancy(r=0.66,P<0.001). Conclusions:KLF8 immunoreactivity is primarily located in trophoblast layer especially in the invasion-related cyto-trophoblast cells. Besides,its expression has a forward association with MMP-9 levels and trophoblast invasion. These results indicate that KLF8 may play an important role in the regulation of trophoblast invasion during implantation and placentation.
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