引用本文:黄 静,孙大光,张 益,杨 柳,计 垣,杨继高,李 红.Notch1在先兆子痫胎盘组织中的表达[J].重庆医科大学学报,2013,(10):1159~1162
Notch1在先兆子痫胎盘组织中的表达
Expression of Notch1 in pre-eclampsia complicated placentas
DOI:
中文关键词:  Notch1  先兆子痫  胎盘
英文关键词:Notch1  pre-eclampsia  placenta
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黄 静,孙大光,张 益,杨 柳,计 垣,杨继高,李 红 重庆市人口和计划生育科学技术研究院出生缺陷与生殖健康重庆市重点实验室重庆 400020 
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中文摘要:
      目的:探讨Notch信号通路成员Notch1在人类先兆子痫发生机制中的作用。方法:获取正常妊娠胎盘组织和先兆子痫胎盘组织,分别采用real-time PCR法、Western blot法、免疫组织化学法检测两种胎盘组织中Notch1 mRNA、蛋白表达及其分布水平。结果:Notch1在两种胎盘组织中均有表达,且主要集中在细胞膜上表达。Notch1在先兆子痫胎盘组织(实验组)的表达水平明显低于正常胎盘组织(对照组)(P<0.05)。结论:推测Notch1可通过Notch信号通路介导胎盘的血管发生、血管形成,影响胚泡滋养层细胞血管内的侵袭过程,其表达下调与先兆子痫的发生有关。
英文摘要:
      Objective:To explore the role of the Notch signaling members Notch1 in the mechanism of human pre-eclampsia. Methods:Placental tissues of normal pregnancy and pre-eclampsia were obtained respectively. Real-time PCR,Western blot and immunohisto-chemical method were used to detect the distribution and expression levels of Notch1 mRNA and Notch1 protein in both placental tissues. Results:Notch1 was expressed in both placental tissues and expression was localized in the membrane. Expression levels of Notch1 in placental tissues of pre-eclampsia(experiment group) were significantly lower than those in placental tissues of normal pregnancy(control group)(P<0.05). Conclusions:Notch1 may mediate the occurrence and development of placental vasculogenesis and influence the endovascular invasion of embryonic cytotrophoblasts through the Notch signaling pathway and the reduced expres-sion level of Notch1 may be associated with the occurrence of pre-eclampsia.
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