引用本文:严秀蕊,王立斌,李玉奎,杨银学.结直肠癌组织中miRNA的差异表达研究[J].重庆医科大学学报,2014,38(3):295~298
结直肠癌组织中miRNA的差异表达研究
Differential expression of microRNAs in human colorectal cancer tissues
DOI:
中文关键词:  miRNA芯片  结直肠癌  microRNAs
英文关键词:miRNA array  colorectal cancer  microRNAs
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严秀蕊,王立斌,李玉奎,杨银学 宁夏医科大学总医院宁夏人类干细胞研究所银川 750004 
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中文摘要:
      目的:寻找并鉴定在结直肠癌及癌旁正常组织中差异表达的miRNA,为进一步阐明其在结直肠癌发生、发展机制中的作用奠定实验基础。方法:收集10例新鲜的结直肠癌及癌旁正常组织,抽提总RNA,利用miRNA芯片技术寻找结直肠癌及癌旁正常组织中差异表达的miRNA,并通过real-time PCR技术进行验证。结果:与结直肠癌旁正常组织相比,结直肠癌组织中上调2.3倍以上的miRNA有32个,下调2倍以上的miRNA有10个;real-time PCR结果与miRNA芯片结果较吻合。结论:筛选得到结直肠癌miRNA差异表达谱,其可能与结直肠癌的发生、发展相关。
英文摘要:
      Objective:To identify the differentially expressed microRNAs(miRNAs) in human colorectal cancer tissues and to make further study on the function of miRNA in pathogenesis and development mechanism of colorectal cancer. Methods:Total RNA was extracted from 10 samples fresh colorectal cancer tissues and normal adjacent tissues and the differentially expressed miRNAs were detected by miRNA array. Real-time PCR was applied to verify the reliability of miRNA array results. Results:miRNA array results showed that 32 miRNAs were up-regulated more than 2.3 folds and 10 miRNAs were down-regulated more than 2 folds in colorectal cancer tissues compared with those in normal adjacent colorectal tissues. And the real-time PCR results were in accordance with the miRNA array results. Conclusion:The miRNA differential expression profile of colorectal cancer is obtained,which might relate with the pathogenesis and development of colorectal cancer.
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