引用本文:李鹍鹏,刘胜春.ERRα mRNA及蛋白在乳腺癌中的表达及其临床意义[J].重庆医科大学学报,2014,38(3):324~327
ERRα mRNA及蛋白在乳腺癌中的表达及其临床意义
mRNA and protein expression of estrogen receptor-related receptor α in breast cancer and its clinical significance
DOI:
中文关键词:  雌激素受体相关受体α  乳腺癌  免疫组织化学  RT-PCR  Western blot
英文关键词:estrogen receptor-related receptor α  breast cancer  immunohistochemistry  reverse transcription-polymerase chain reac-tion  Western blot
基金项目:
作者单位
李鹍鹏,刘胜春 重庆医科大学附属第一医院内分泌乳腺外科重庆 400016 
摘要点击次数: 630
全文下载次数: 447
中文摘要:
      目的:研究雌激素受体相关受体α(estrogen receptor-related receptor α,ERRα)mRNA和蛋白在乳腺癌组织及乳腺癌旁组织的表达及意义,进一步探讨ERRα与乳腺癌临床病理指标[雌激素受体(estrogen receptor,ER)、孕激素受体(progesterone re-ceptor,PR)、人表皮生长因子受体-2(human epidemic growth factor receptor-2,Her-2)、P53],以及与乳腺癌分级、分期的相关性。方法:收集乳腺癌及对应乳腺癌旁组织标本87例,分别采用免疫组织化学染色,RT-PCR,Western blot检测ERRα mRNA和蛋白的表达情况,进一步分析ERRα的表达与乳腺癌临床病理特征的关系。结果:本研究中ERRα mRNA和蛋白在乳腺癌组织中的表达均高于在乳腺癌旁组织中的表达(P<0.05),且ERRα的表达与乳腺癌病理指标Her-2、ER以及肿瘤的病理分级,肿瘤大小,淋巴结转移相关,而与患者的年龄、PR、P53等无明显相关。结论:ERRα在乳腺癌中的高表达,可能作为乳腺癌一个预后不良的新的分子标记物,为乳腺癌的诊治提供新的思路。
英文摘要:
      Objective:To detect the expression and significance of estrogen receptor-related receptor(ERR) α mRNA and protein in breast cancer and breast para-carcinoma tissues and to further discuss the correlation between ERR α and breast cancer clinical pathological index((estrogen receptor(ER),progestin receptor(PR),human epidermal growth factor receptor-2(Her-2),P53) as well as the grade and classification of breast cancer. Methods:Expression of ERRα in 87 breast cancer tissues and the corresponding breast para-carcinoma tissues were detected by immunohistochemistry and reverse transcription PCR(RT-PCR),Western blot respec-tively and their relationship with the clinical pathological characteristics was analyzed. Results:Expression of ERR α mRNA and pro-tein in breast cancer tissues was higher than that in breast para-carcinoma tissues(P<0.05). Expression of ERR α was correlated with ER,Her-2,degrees of differentiation,tumor size and metastasis of the lymph nodes but was not correlated with patient’s age,PR and P53. Conclusions:High expression of ERR α may serve as a biomarker of poor prognosis in breast cancer and it may provide new thought for the diagnosis and treatment of breast cancer.
查看全文  查看/发表评论  下载PDF阅读器
关闭
微信关注二维码