引用本文:周小军1,陈江华2,王士贞3,王书芹4,张丽娟1.鼻咽癌癌变前后ADAM23、MIMT1、FAM150B、GRIN2A、LRRC4甲基化研究[J].重庆医科大学学报,2014,38(3):341~344
鼻咽癌癌变前后ADAM23、MIMT1、FAM150B、GRIN2A、LRRC4甲基化研究
Study on methylation of ADAM23,MIMT1,FAM150B,GRIN2A,LRRC4 before and after nasopharyngeal carcinoma canceration
DOI:
中文关键词:  鼻咽癌  DNA甲基化  ADAM23  MIMT1  FAM150B  GRIN2A  LRRC4
英文关键词:nasopharyngeal carcinoma  DNA methylation  ADAM23  MIMT1  FAM150B  GRIN2A  LRRC4
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作者单位
周小军1,陈江华2,王士贞3,王书芹4,张丽娟1 1. 广州中医药大学附属中山市中医院耳鼻咽喉科中山 5284002. 广州中医药大学附属中山市中医院检验科中山 5284003. 广州中医药大学第一临床医学院耳鼻咽喉科广州 5104054. 广州中医药大学附属中山市中医院病理科中山 528400 
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中文摘要:
      目的:研究鼻咽癌变前后ADAM23、MIMT1、FAM150B、GRIN2A、LRRC4甲基化特征。方法:12例鼻咽癌10例鼻咽炎作为研究对象,抽提鼻咽组织DNA,以特异识别5-甲基胞嘧啶的抗体免疫沉淀DNA,实时荧光定量PCR分别检测5个基因含量,并设置不用5-甲基胞嘧啶抗体(非MeDIP-qPCR实验)及使用非特异性抗体IgG进行免疫沉淀作为双重对照。结果:无论鼻咽癌还是鼻咽炎患者,5个基因在使用非特异性抗体IgG实验时均未检验到甲基化,非MeDIP-qPCR实验显示5个基因在鼻咽癌及鼻咽炎荧光定量值无明显差异(P >0.05);MeDIP-qPCR实验显示,鼻咽癌5个基因均呈现明显的甲基化,ADAM23、MIMT1、FAM150B、GRIN2A、LRRC45在鼻咽癌校正值分别为:3.39±2.28、6.03±3.93、3.68±1.81、7.12±3.17、3.10±1.94,在鼻咽炎分别为0.00±0.00、0.01±0.01、0.25±0.49、0.20±0.20、0.00±0.00(均P<0.01)。结论:ADAM23、MIMT1、FAM150B、GRIN2A、LRRC4 5个基因甲基化为鼻咽癌变的重要特征。
英文摘要:
      Objective:to study ADAM23,MIMT1,FAM150B,GRIN2A,LRRC4 DNA methylation feature while nasopharyngeal epithe-lium canceration. Methods:Totally 12 patients with nasopharyngeal carcinoma and 10 patients with nasopharyngitis were chose as the research objects. Nasopharyngeal tissue DNA was extracted and was used to specifically identify 5-methyl cytosine antibody immune precipitation DNA. Real-time fluorescent quantitative PCR was used to detect the contents of five genes respectively. Non 5-methyl cytosine antibody(non MeDIP-qPCR experiment) and nonspecific antibody IgG immune precipitation were set up as dual controls. Results:Regardless of the patients with nasopharyngeal carcinoma or nasopharyngitis,methylation was not detected in five genes when using nonspecific antibody IgG experiment. Non MeDIP-qPCR experiment showed that there was no difference in fluorescence quan-titative value of five genes in nasopharyngeal carcinoma and nasopharyngitis(P >0.05). MeDIP-qPCR experiment showed that all five genes in nasopharyngeal carcinoma presented significant methylation. Corrected values of real-time fluorescence quantification of ADAM23,MIMT1,FAM150B,GRIN2A,LRRC45 were 3.39±2.28,6.03±3.93,3.68±1.81,7.12±3.17,3.10±1.94 in nasopharyn-geal carcinoma and were 0.00±0.00,0.01±0.01,0.25±0.49,0.20±0.20,0.00±0.00 in nasopharyngitis(all P < 0.01). Conclusion:ADAM23,MIMT1,FAM150B,GRIN2A,LRRC4 gene methylation are important characteristics of nasopharyngeal carcinoma.
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