引用本文:崔欢欢1,李继斌2,柴水琴1,明 月1,王冬娟3,彭 川2,刘 丹3,肖晓秋1.ob/ob肥胖小鼠肝脏炎症及能量代谢相关转录因子表达特征[J].重庆医科大学学报,2014,38(7):915~918
ob/ob肥胖小鼠肝脏炎症及能量代谢相关转录因子表达特征
Characteristics of expression of energy metabolism-related transcription factors in the liver of ob/ob mice
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中文关键词:  ob/ob小鼠  脂肪肝  炎症  过氧化酶体增殖物激活受体γ共激活因子-1α  过氧化物酶体增殖物激活受体α  核呼吸因子1
英文关键词:ob/ob mice  fatty liver  inflammation  peroxisome proliferator activated receptor γ coactivator-1α  peroxisome proliferator activated receptor α  nuclear respiratory factor 1
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崔欢欢1,李继斌2,柴水琴1,明 月1,王冬娟3,彭 川2,刘 丹3,肖晓秋1 1. 重庆医科大学附属第一医院脂糖代谢实验室重庆 4000162. 重庆医科大学公共卫生与管理学院营养与卫生学教研室重庆 4000163. 重庆医科大学附属儿童医院、儿童发育疾病研究省部共建教育部重点实验室重庆 400014 
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中文摘要:
      目的:以ob/ob基因敲除肥胖小鼠为研究模型,探讨肥胖对小鼠肝脏炎症的影响以及ob/ob小鼠肝脏中与能量代谢相关转录因子:过氧化物酶体增殖物激活受体γ共激活因子-1α(peroxisome proliferator activated receptor γ coactivator-1α,PGC-1α)、过氧化物酶体增殖物激活受体α(peroxisome proliferator activated receptor α,PPARα)、核呼吸因子1(nuclear respiratory factor 1,NRF1)的基因表达特征。方法:选取以C57BL/6J为背景的雄性ob/ob肥胖小鼠(以下简称ob/ob小鼠)作为肥胖模型,同龄正常C57BL/6J雄性小鼠作为对照,2组小鼠各8只,于SPF级动物房饲养至5月龄,处死后取肝组织,石蜡包埋切片,HE染色观察组织形态;抗原F4/80免疫组化染色观察巨噬细胞浸润情况;RT-PCR检测各转录因子mRNA表达水平。结果:5月龄ob/ob小鼠体质量和肝脏质量高于对照组(t=9.66,P=0.000;t=9.98,P=0.000);石蜡切片HE染色结果显示ob/ob小鼠肝细胞胞浆内出现空泡状脂肪滴;F4/80免疫组化结果显示ob/ob小鼠肝脏组织巨噬细胞浸润明显;RT-PCR结果显示ob/ob小鼠肝脏组织转录因子PGC-1α、PPARα、NRF1的mRNA表达水平较对照组明显上调(t=3.02,P=0.009;t=5.64,P=0.000;t=2.93,P=0.011)。结论:ob/ob小鼠表现出显著的肥胖特征,肝细胞出现明显脂质沉积,肝组织炎性细胞浸润;上述改变可能与肝脏能量代谢相关转录因子PGC-1α,PPARα和NRF1的表达增加有关。
英文摘要:
      Objective:To examine the mRNA expression characteristics of hepatic energy metabolism-related transcription factors:peroxisome proliferator activated receptor γ coactivator-1α(PGC-1α),peroxisome proliferator activated receptor α (PPARα) and nuclear respiratory factor 1(NRF1) in ob/ob mice. Methods:Male ob/ob obese mice on a C57BL/6J genetic background were used as obese model(n=8) and normal male C56BL/6J mice as control(n=8). At the age of 5 months,liver samples were obtained after sacri-ficing. Liver morphologic analysis was performed by HE staining and macrohoages were stained by immunohistochmistry method us-ing F4/80 antibody. RT-PCR was performed to determine the mRNA expression levels of interested genes. Results:Both body weight and liver weight of the ob/ob mice were higher than those of control group(t=9.66,P=0.000;t=9.98,P=0.000). In the ob/ob group,HE showed lipid accumulation. Immunohistochmistry staining for F4/80 results showed more severe inflammatory cell infiltration in ob/ob group. Compared with those in control group,PGC-1α,PPARα,NRF1 mRNA expression levels increased significantly(t=3.02,P=0.009;t=5.64,P=0.000;t=2.93,P=0.011). Conclusion:The ob/ob mice are obese than normal controls. There are significant hepatic lipid accumulation and inflammatory cell infiltration in the livers of ob/ob mice,which may related with the increased expressions of PGC-1α,PPARα and NRF1.
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